<br/>Experiments simulating the intracellular environment often neglect to include the high concentration of macromolecules within the cytosol of the cell. The influence of these macromolecules is a crowding effect, which impacts the rate at which reactions occur within the cell. Utilizing the methods of Brownian Dynamics, simulations were used to calculate the protein-protein association rate of the Ferredoxin NADP+ Reductase (FNR) with the corresponding Ferredoxin (Fd) under standard conditions as well as those including macromolecular crowding. The resulting data indicated that the diffusion-controlled rate of the reaction was reduced when introduced to crowded conditions. This simulation and data indicates a greater understanding of the interactions occurring within a realistic model of the intracellular environment.

Effects of viscosity and macromolecular crowding on the diffusion-controlled rate constant of ferredoxin with ferredoxin NADP<sup>+</sup> reductase

251st National Meeting (2016)

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Effects of viscosity and macromolecular crowding on the diffusion-controlled rate constant of ferredoxin with ferredoxin NADP+ reductase

Effects of viscosity and macromolecular crowding on the diffusion-controlled rate constant of ferredoxin with ferredoxin NADP⁺ reductase