The majority of infectious diseases are initiated by the adhesion of pathogens and toxins to the host tissues. Agents that can disrupt or inhibit pathogens and toxins attachment to the host tissues, and thus reduce the incidence of infectious diseases are alternatives to antibiotics, which are known the cause of drug-resistant bacteria.<br/>Ovomuicn, a glycoprotein accounting approximately for 2-4% of the total egg albumen protein, is a member of mucin family and consists of 33% of carbohydrate content on average. Ovomucin is rich in sialic acids (2.6-7.4%, w/w), which are a family of acylated derivatives of a nine-carbon carboxylated monosaccharide and play important roles in bacteria adhesion (act as glycan receptor on the surface of host tissues). The objective of the study was to study the potential of ovomucin derived peptides as an anti-adhesive agent.<br/>Ovomucin was hydrolyzed by 14 kinds of proteases and their anti-adhesive activities were screened using the hemagglutination assay. Nine hydrolysates showed minimum inhibitory concentrations (MIC) ranging from 10 to 0.31 mg/mL for two Enterotoxigenic <i>Escherichia coli</i> strains (ECL13795 and ECL13998). Ovomucin acid protease II hydrolysate showed the best anti-agglutinating activity (MIC: 0.31 mg/mL), and was chosen to further fractionate by ion exchange chromatography and reverse-phase high performance liquid chromatography. Finally, two fractions exerted the most potent activity, with MIC of 0.25 mg/mL and 0.03 mg/mL against strain 1033 and 1032, respectively, were further studied in IPEC-J2 cells (porcine small intestinal epithelial cell line) and characterized by HPLC-MS. Ovomucin derived peptides may have potential application as an anti-adhesive agent to prevent the infectious diseases.

Ovomucin derived peptides as anti-adhesive agents against infectious diseases

251st National Meeting (2016)

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