Short interfering RNAs (siRNAs) specifically knock down genes when delivered to cells by triggering an RNA-interference response (RNAi). Currently, the cellular uptake of siRNA remains a great challenge since their highly negative charge impedes penetration of cell membranes. We have developed a method to generate siRNA-polymer amphiphiles that self-assemble into spherical micelles with a diameter of ca. 20 nm. We show how this <i>micellar siRNA</i> is taken up by cells without the need for toxic transfection reagents. Furthermore, we provide evidence for the efficient and specific downregulation of gene expression levels in HeLa cells. The described RNA-polymer amphiphile nanoparticles represent a novel class of soft nanomaterials that is capable of interfering with biological systems in a predictable manner.

Self-transfecting micellar siRNA

251st National Meeting (2016)

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