The enzyme immobilization is very important in medical, food industry and other sectors due to the enormous application in process such as antibacterial uses, chemical reactions and other ones. The most inert common support matrixes are nanoparticles and polymers, being the most common on polymers. The means to carry out enzyme immobilization are covalent bonds, inclusion, adsorption, absorption, crosslink between others ones.<br/>Therefore, the goal of this work was to carry out the lysozyme immobilization through formation of covalent bonds on silicone rubber (S-R) previously modified with EGDMA/GMA graft using gamma-rays and azobisisobutyronitrile (AIBN) as initiators, and followed by chemically reactions, firstly by cyanide and afterwards by a reduction to generate an amine, where the enzyme was immobilized using glutaraldehyde.<br/>The characterization was performed by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and infrared (FTIR-ATR) spectroscopy, finally the activity of immobilized enzyme was measured using micrococcus lysodeikticus as a standard test at different pHs and temperatures.

Lysozyme immobilization on silicone rubber modified with EGDMA/GMA graft started by radiation and free radicals

251st National Meeting (2016)

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