The cellulose nanofibers were successfully prepared by the laccase-TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidation system and the following ultrasonic treatment from HBKP (hardwood bleached kraft pulp). The cellulose was dispersed in buffer solution with laccase and TEMPO for several days. During the oxidation, O2 was constantly pumped in so as to make sure that the oxidation sufficiently proceeded. With other substrates of laccase added in, the efficiency of the oxidation was improved resulting from the synergistic reaction. The amount of other substrates was much less than TEMPO at a ratio of 1:500. The most efficient one was PSPX (phenoxazine), the correspondent carboxylate content of oxidized cellulose was higher than the cellulose oxidized by original laccase-TEMPO system, which were 0.65 mmol/g and 0.58 mmol/g, respectively. Although the other substrates didn`t worked so well, such as HPI (N-hydroxyphthalimide) and ABTS (2,2`-azino-bis(3-ethylbenzothiazoline-6sulfonic), the efficiency of these oxidations also improved. At the same time, all the oxidized cellulose were transformed to nanofiber dispersions （Fig.1). There were no obvious difference between the nanofibers in morphology, thermal stability and rheology. By the way, the detailed reactions of the oxidation were not clear, which required deeper researches. Acknowledgements This research was supported by the National Forestry Public Welfare Industry Research Project (201304609), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (12KJA220001), the Doctorate Fellowship Foundation of Nanjing Forestry University

Effect of synergistic reaction on the O2/laccase/TEMPO oxidation of cellulose and the preparation of nanofibers

251st National Meeting (2016)

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