<br/>A new class of broadly neutralizing antibodies (bNABs) from HIV donors has been reported to target the glycans on gp120 thus renewing hope of developing carbohydrate- based HIV vaccines. The gp120 used in the studies was, however, not from the host human T cells and so the glycosylation patterns could be different, and some of the antibodies recognized two different glycans simultaneously which could not be detected on the commonly used N-hydroxysuccinimide (NHS) glycan microarray. Here, we developed a glycan microarray on an NHS- or aluminium oxide-coated glass slide (ACG) containing a diverse set of glycans, including homo- and mixed N - glycans of high- mannose-, hybrid-, and complex-type that were prepared by modular chemo- enzymatic methods to detect the presence of hetero-glycan binding behaviors. This new approach allows rapid screening and identification of optimal glycans recognized by neutralizing antibodies which could speed up the development of HIV-1 vaccines targeting cell surface glycans.

Modular synthesis of N-glycans for homogeneous and mixed-glycan arrays to study hetero-ligand binding of HIV-1 broadly neutralizing antibodies

251st National Meeting (2016)

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