Exosomes hold promising potential for cancer research and clinical diagnosis. The progress in the field, however, is severely hampered by the technical challenges in isolation and analysis of exosomes. Here we developed a microfluidic exosome analysis platform functionalized with a bioinspired polydopamine-graphene oxide (PDA-GO) nano-interface. We showed that this nano-interface markedly improves the efficiency of exosome immuno-capture while effectively suppressing the effects of non-specific interactions and fluorescence quenching by GO. Based on this platform, an ultrasensitive exosome ELISA assay was developed to afford a low detection limit of 50 mL<sup>-1</sup> with a 4-log dynamic range, which are orders of magnitude better the existing methods. We demonstrated the ability of this platform to discriminate ovarian cancer patients from healthy controls by sensitive, specific and rapid detection of targeted exosomes using 2 μL patient plasma without the need of sample processing. Thus, this platform could provide a useful tool to facilitate exosome research and clinical utilities for disease detection and treatment.

Bioinspired polydopamine-graphene oxide nano-interface enables ultrasensitive microfluidic analysis of exosomes

251st National Meeting (2016)

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