Our standard cell culture platform technology for synthesis of therapeutic proteins is fed-batch processes using Chinese hamster ovary (CHO) cells. Advances in cell line and process development have enabled achievement of high titers with these platform cell culture processes. Cell culture processes are generally simplified when needed to meet the imperatives of fast timelines for early development and also for manufacturing-friendliness. However, process parameters, composition of the basal and feed media and feeding strategies need to be optimized for specific cell lines when there is a need to achieve increased titers to meet greater product demand. This poster will focus on the specific enhancements that were made to the platform process for achieving much higher titers. A case study will be used to demonstrate the potential for achieving higher productivity when feeding strategy, media composition, and other process parameters are optimized. It is important to ensure that these process changes do not adversely impact product quality and strategies used to control the acidic charge variant profile will be discussed.

Strategies for optimizing a cell culture platform to achieve high recombinant protein titer

251st National Meeting (2016)

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