The determination of proteins, peptides, DNAs and small molecules is of considerable importance in clinic diagnosis and therapy, bioassays, drug designs, environmental monitoring and food safety assessment. The development of strategies combined high sensitivity, selectivity with simplicity and generalization for determining proteins, peptides, DNAs and small molecules are highly desired. Herein, we report a general label-free chemiluminescence (CL) strategy for biosensing of proteins, peptides, DNAs and small molecules based on that target could enhance or inhibit the CL produced by the reaction of N-(4-Aminobutyl)-N-ethylisoluminol (ABEI) functionalized gold colloids1 with H2O2. Firstly, recognition probe such as aptamer, antibody, single-strand DNA and agglutinin was immobilized to a SA-coated microwell. Then targets were captured by recognition probe. Subsequently, a portion of ABEI-Au colloids as signal reporters was added to the microwell. When an aliquot of H2O2 was injected into the microwell, the CL signal was generated. The strategy could be used for the determination of thrombin, human IgG, arginine vasopressin, specific DNA sequences from tuberculosis, ATP, TNT, tetracycline, dopamine and mannose with the detection limit of 0.93 nM-4.1 fM.2 This work provides a general, simple, sensitive and selective strategy for biosensing of proteins, peptides, DNAs and small molecules, which is of great application potential in clinical diagnosis, pharmaceutical analysis, environmental monitoring and food safety assessment. The support of this research by the National Natural Science Foundation of China (Grant Nos. 21075115 and 21173201) and the Opening Fund of State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, CAS (grant no. SKLEAC201408) are gratefully acknowledged. Reference: D.Y. Tian, Y.Chai, H.L. Zhang, H. Cui, Chem. Commun., 2011, 47, 4959-4961. S.Y. Li , D.Y. Chen , Q.T. Zhou , W. Wang , L.F. Gao , J. Jiang , H.J Liang , Y.Z. Liu , G.L. Liang and H. Cui, Anal. Chem. 2014, 86 (11), 5559–5566.

General chemiluminescence strategy for biosensing of proteins, peptides, DNAs and small molecules

251st National Meeting (2016)

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