<b>Abstract: </b>Modern technology has generated a large number of techniques for identifying pathogenic organisms and diagnosing diseases, but many commonly used diagnostic procedures—including ELISA and PCR—are expensive and must be performed by trained laboratory personnel, which limits their usefulness in rural, developing-world, and home-user applications. Cheaper diagnostic techniques, such as the lateral flow assay and the microfluidic paper-based analytical device, are much more useful in these settings but have other limitations; the lateral-flow assay, for example, generally requires high sample concentrations, while the microfluidic paper-based analytical device depends on the presence of enzymes in the analyte.  By leveraging the affinity of proteins for nitrocellulose (as in Western blotting) and the sensitivity of fluorescent labeling, we have developed a prototype biosensor that combines the strengths of lateral-flow and microfluidic paper-based devices into a “microfluidic immunoassay biosensor” that reliably detects the presence of antibodies in a sample of only 30 µL volume.  This type of device can be produced cheaply and operated by an untrained user even when only small amounts of analyte are available. <br/>

Prototype microfluidic immunological biosensor for point-of-care diagnostics

251st National Meeting (2016)

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