A new reversed-phase high-performance liquid chromatographic method utilizing fluorescence detection after post-column derivatization with monobromobimane (mBrB) is described for the determination of homocysteine, cysteine, cysteinyl-glycine, and glutathione in plasma and tissue. Comparison of accuracy, precision, and limit-of-detection are compared to an existing HPLC method coupled with pre-column derivatization. Improvement in sample throughput and reduced sample preparation over existing methods is also assessed and presented. The optimized method was demonstrated with the determination and comparison of the aminothiols in normal mice and those that express Down’s Syndrome.

Determination of aminothiols in blood and tissue by high performance liquid chromatography coupled with post-column derivatization and fluorescence detection

251st National Meeting (2016)

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