Nanopore technology has emerged as a powerful approach to make a nanosensor to detect the interactions between the analyte and membrane protein at single molecule level. Recent studies in our group have employed biological nanopores to discriminate single oligonucleotides, peptides, and proteins, which includes the detection of their conformational changes, monitoring the interactions of an ATP and aptamers, enhanced translocation of poly(dT) by introducing a Fab fragment and investigate the self-assembly of oligonucleotide–peptide conjugates[1-4]. We also designed a calixarene functionalized biological nanopore to real-time monitor the light-regulated host-guest interactions by monitoring the characteristic signals, and analysis of the light-regulated RNA:spiropyran interactions at single molecule level[5-6]. [1] Ying Y L, Zhang J, Gao R, et al. Angew. Chem. Int. Ed. 2013, 50(52): 13152. [2] Ying Y L, Wang H Y, Sutherland T C, et al. Small. 2011, 7(1), 87. [3] Ying Y L, Li D W, Li Y, et al. Chem. Commun. 2011, 47, 5690. [4] Ying Y L, Li D W, Liu Y, et al. Chem. Commun. 2012, 48, 8784. [5] Ying Y L, Zhang J, Meng F N, et al. Sci. Rep. 2013, 3. [6] Zhang X, Zhang J, Ying Y L, et al. Chem. Sci. 2014, 5(7): 2642.

Single molecule analysis by biological nanopores

251st National Meeting (2016)

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