Accurate, sensitive and rapid detection of panel of biomarkers related to cancer can show significant impact in personalized cancer therapy. Point of care diagnostics often face challenges being sensitive, low cost and automated. Herein, we report a low cost, microprocessor controlled automated 3D printed electrochemiluminescent (ECL) arrays to detect a panel of 7 prostate cancer biomarkers in serum samples. Using Form 1+ 3D printer we printed a unibody optically clear ECL microfluidic array with 5 reagent reservoirs that can hold Serum, wash buffers, ECL label and tripropylamine. These sample/reagents are delivered to common microfluidic serpentine channel for detection of multiple proteins in serum simultaneously. An under lying pyrolytic graphite chip patterned with microwells and decorated with capture antibody coated single wall carbon nanotube forest was used as sensor surface for sensitive detection. Complete automation of sample and reagent delivery is achieved by incorporating Arduino based micro pump system. Onboard integrated supercapacitors drive all micropumps and ECL generation, generated ECL was captured by a digital camera makes it portable for resource poor setting. Ultralow levels of these biomarkers were achieved in serum within 40 minutes.

3D-printed, automated and low cost ECL arrays for detection of multiple prostate cancer biomarkers

251st National Meeting (2016)

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