Asymmetric Flow Field-Flow Fractionation (AF4) combined with multidetector analysis form a promising technique for nanoparticle characterization. In this work we optimized the technique to characterize the interaction between an archetypal antimicrobial peptide and synthetic membranes [1]. By using charged and neutral liposomes it is possible to mimic some of the charge characteristics of biological membranes. The use of AF4 system allows determining, in a single analysis, information regarding the selectivity of the peptides, the quantity of peptides bound to each liposome, the induced change in the size distribution and morphology of the liposomes. The results obtained provide relevant information for the study of structure-activity relationships in the context of membrane-induced antimicrobial action. This information will contribute to the rational design of potent antimicrobial agents in the future. References 1. P. Iavicoli et al. Application of Asymmetric Flow Field-Flow Fractionation Hyphenations for Liposome-Antimicrobial Peptide Interaction. J.Chromatog. A (2015). http:// 10.1016/j.chroma.2015.10.029

Flow field-flow fractionation hyphenations for liposome-antimicrobial peptide interaction

251st National Meeting (2016)

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