A gold based nanosensor for easy, accurate and sensitive detection of Lutenizing Hormone (LH) in sheep’s blood has been developed. The sensor utilizes lateral flow technique to detect LH directly from sheep blood. Reproduction ability of sheep depends on concentration of LH in blood. In the present study, the capability of sensor for LH detection during the ovulation period of sheep (n =5) was evaluated. Blood was withdrawn from the study animals on different time intervals and used for analysis. Results from the analysis of blood samples demonstrate the sensor can detect as low as 50 ng of LH. The results obtained have been compared with the traditional radioimmunoassay procedures. Field testing results are highly reproducible. To further increase the analytical capability, magnetic nanoparticles were developed to remove LH selectively from the blood. The enriched sample was analyzed for LH. The poster present the objectives, results, outcome and future directions of the sensor study.

Field evaluation of gold based nanosensor in a local sheep farm

251st National Meeting (2016)

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