Efficiency can be used to quantitatively compare different experimental setups for chromatography or other separation processes. To better understand the factors that affect efficiency, it is important to study the interactions between analytes, mobile phases, and stationary phases. The separation performance of various mobile phase consisting of mixtures of aqueous buffers or formic acid with different amounts of acetonitrile or methanol were studied by capillary electrochromatography (CEC) on a lauryl acrylate porous polymer monolith column. In addition, chromatographic figure of merits were also calculated for columns prepared from different polymerization recipes by using two distinct cross-linkers, 1,3-butanediol diacrylate (BDDA) and trimethylpropyl trimethacrylate (TMPTMA). Cross sections of these columns were sputter coated with gold and imaged under scanning electron microscope (SEM). Quantitative comparison between columns was enabled from adjusted SEM measurements. The morphological discoveries provide explanations for the column performance. The results of this study also provide insights for understanding the relationship between the structure and property of lauryl acrylate porous polymer monoliths.

Efficiency study of porous polymer monoliths with different mobile phase and stationary phase compositions using capillary electrochromatography (CEC) and scanning electron microscope (SEM)

251st National Meeting (2016)

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