In single cell analysis, developing high throughput microfluidic devices is essential for fast, continuous and efficient cell analysis. Single cell transport using pressure/ vacuum driven flow, always offers relatively slow analyzing rates. Many previously reported techniques provide cell transporting rate up to 25-40 cells/h. In such techniques, flow rates are less controllable and small precise adjustments cannot be made. In order to improve the throughput of the devices, we have introduced high throughput fluid transport using multilayer softlithographic valves. These valves were utilized as on-chip peristaltic pumps and throughput was increased up to ~1000 cells/h. Pneumatically controlled nine micro-valves were used to continuously pump the fluid in automated fashion. These pumps are simple, durable, easy to fabricate and bio-compatible. At the maximum performance, pumps produce flows up to 9.2nL/s. T-lymphocytes (Jurkat cells) were labeled with 6-carboxyfluirescein (6-CFDA) and Oregon green (OG). Peristaltic pumps were used to drive the cells from sample reservoir to waste reservoirs. Cells were electrically lysed and electrophoretically separated using same electric field. Separation of CFDA and OG were detected using laser induced fluorescent (LIF) detection system.

High throughput single cell analysis using multilayer microfluidic devices

251st National Meeting (2016)

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