The protein encoded by the Streptomyces griseus gene desIV is believed to catalyze the second step in the pathway that leads to the production of the antibiotic streptomycin. The goals for this project are to clone desIV and characterize the protein's function. Initial cloning attempts revealed that desIV had not been successfully inserted. Rather, the insert was a primer dimer that resulted from a five nucleotide match between the forward and reverse primers. A new reverse primer was used to eliminate the primer dimer. Progress towards cloning and characterization of desIV will be reported.

Cloning desIV

251st National Meeting (2016)

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