A Gram-positive marine bacterial strain, UA 826, was isolated from near-shore sediment collected at Swami’s State Marine Conservation Area in Cardiff-by-the-Sea, CA. The sample was plated on media selective for Bacillus species. On 10L culture and extraction of the culture broth, the mid-polarity fractions were found to be interesting both chemically via 1H NMR analysis and biologically via a fluorescence-based growth assay for an auxotroph of Mycobacterium marinum, the closest genetic relative of M. tuberculosis. The polar and non-polar organic components were found to be the metabolites typical of marine bacterial cultures and showed no growth inhibition in the M. marinum assay. Fractionation by flash chromatography resulted in several interesting components that are currently being purified and identified.

Anti-mycobacterial metabolites from a Gram-positive marine bacterium

251st National Meeting (2016)

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