With the global increase of multidrug-resistant TB, increased focus on the purification and characterization of new antimicrobials is urgently needed. The marine environment is relatively unexplored in terms of potential pharmaceuticals. This study examines the metabolites of a marine bacterium, strain UA-461, which was shown to inhibit the growth of Mycobacterium marinum, a close genetic relative of M. tuberculosis. The extracts had peaks of interest in the NMR spectra that could potentially be the source of the biological activity. The bacterium was grown on a 10L scale, centrifuged, filtered, then placed on a C-18 column. Elution was performed with 1:1 methanol:water (F1), followed by methanol (F2) and then ethyl acetate (F3). NMR analysis showed the components in the mid-polarity range to contain the interesting material, therefore further purification of these components using HPLC is underway.

Isolation of a natural product from a marine bacterial culture challenged with Mycobacterium marinum

251st National Meeting (2016)

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