A copper sulfide immunoconjugate nanoparticle will allow the antibody to carry a larger payload to the target site compared to the traditional bifunctional chelate. Nanoparticles are synthesized via direct reaction with copper(II) chloride and sodium sulfide and capped with citrate. The surface is functionalized with lipoic acid PEG.  Antibodies are conjugated to the nanoparticle with an EDC/NHS reaction and purified with gel filtration. The core nanoparticles have an average size of 8.8 ± 1.4nm. Radiolabeling studies with copper-64 showed a radiochemical yield of 81% and the nanoparticle retained 95% of the activity over a 24 hour period. Increased stability in solution and less aggregation upon TEM analysis is indicative of successful PEGylation.

Development of a copper sulfide (CuS) immunoconjugate nanoparticle for targeted radiotherapy

251st National Meeting (2016)

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