Biosensors harness the specificity of biologically relevant receptors in order to detect and study cellular binding. One subset of biosensors is immunosensors, which rely on antibody-antigen interactions. Immunosensors can be used in both research and clinical settings to detect antibodies, and determine if a host or patient is infected with a particular disease. Typically, for the antibodies to be detected, the antigen and bound antibodies need to be immobilized on a solid phase. A relatively straightforward way to immobilize the antibody-antigen complex is by using self-assembled monolayers (SAMs) to chemically modify a surface. The SAM tail group can bind to a variety of functional groups, in this case, the antigen complex, and the SAM can act as a molecular bridge between the ligand and the substrate. This study explored methods to prepare the substrate zinc selenide (ZnSe) for successful chemisorption of carboxylate SAMs by removing the oxide layer that forms on the ZnSe surface. Further, both immersion and microcontact printing techniques were used to apply carboxylate SAMs and the antigen complex to the passivated ZnSe surface. The substrate and SAM were characterized using contact angles, infrared spectroscopy, and x-ray photoelectron spectroscopy.

Formation of immunosensors using self-assembled monolayers on zinc selenide

251st National Meeting (2016)

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