Surface-enhanced Raman spectroscopy (SERS) immunoassays have been increasing in popularity in the past several years due the ability to provide low limits of detection, high sensitivity, and multiplexed detection. While SERS-based assays have led to improvements in the analytical performance of immunoassays relative to conventional assays, e.g., ELISAs, greater efforts are needed to develop convenient and rapid SERS-based assays that are suitable for point-of-need applications. To this end, we show the development of a multiplexed immunoassay utilizing antigen-mediation aggregation of gold nanoparticles (AuNP).  We recently developed a SERS-based aggregation immunoassay for one analyte that provided better detection limits and faster analysis when compared to ELISA. This work focuses on the expansion of the assay for multiplexed detection.  This aggregation immunoassay is performed using extrinsic Raman labels (ERLs), which consist of a AuNP modified with a mixed monolayer of a Raman dye and an antibody. In order to obtain a mutiplexed assay, ERLs were made using unique combinations of Raman dye and antibodies specific for mouse IgG, goat IgG, and rabbit IgG. Several different Raman dyes were systematically studied in order to obtain a unique signal for each antigen that is being studied. Cross reaction and nonspecific aggregation of the ERLs were also evaluated to establish proof-of-principle for the multiplexed SERS assay.

Development of a mutiplexed point-of-care SERS immunoassay based on antigen mediated aggregation of gold nanoparticles

251st National Meeting (2016)

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