DNA origami is a technique, in which long single-stranded DNA is folded into a designed nanostructure, in either 2D or 3D, with the aid of many short staple strands [1]. We recently have developed several “dynamic” nanomechanical DNA origami devices using with transformation capability, such as DNA origami pliers and DNA origami boxes [2-4]. DNA origami pliers are the devices that can be used as “single-molecule” beacons to detect a variety of biorelated molecules, from metal ions at the size of a few tens of atomic mass number units to relatively gigantic proteins with a molecular mass greater than a hundred kilodaltons, all on a single platform. On the other hand, gold nanoparticle(AuNP)–DNA hybrids are typical inorganic/organic or bio/non-bio fusion material popularly studied to date, because of the established conjugation technology between AuNPs and DNA [5], and a large pool of various 2D and 3D DNA nanostructures as the fruits of structural DNA nanotechnology based on branched DNA motifs [6]. A box-shaped 3D DNA origami of ca. 40 nm dimensions, which is selectively formed by mechanically closing symmetric open motif with three orthogonal faces [4], was used as an intelligent nano-container to encapsulate exact number of 10-nm gold nanoparticles (AuNPs) (Fig. 1). AuNPs were functionalized with thiol modified DNA strands and attached to one of the face of the open motif, which is designed to be an interior surface of the box, decorated with three complementary strands. The open motif was then closed into the box shape triggered by the addition of DNA strands joining the remaining edges. Atomic force microscope (AFM) and transmission electron microscope (TEM) imaging of agarose-gel purified complexes clearly showed successful encapsulation of one AuNP inside the shell [7]. [1] Rothemund, P. W. K. Nature 2006, 440, 297. [2] Kuzuya, A., Ohya, Y. Acc. Chem. Res., 2014, 47, 1742. [3] Kuzuya, A. et al., Nat. Commun. 2011, 2, 449. [4] Kuzuya, A. et al., Chem. Commun. 2009, 4182. [5] Ohya, Y., Kuzuya, A. et al., Small 2012, 8, 2335. [6] Kuzuya, A., Ohya, Y., Polym. J. 2012, 44, 452. [7] Kuzuya, A.; Ohya, Y. et al., Polym. J. 2015, 47, 177.

Design of nano-mechanical DNA origami devices with dynamic characters

251st National Meeting (2016)

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