Conducting polymers are used as excellent immobilization platform for biomolecules led to the development of efficient biosensors, taking the advantages of ease of processability, ability of conduct the electricity and low cost1. Combining synthetic polymers with polypeptide segments becomes a promising approach in the field of enzyme immobilization since polypeptides segments possess remarkable biocompatability and exhibit a 3D conformation under certain conditions2. Also, development of ferrocene polymer modified electrodes provides superior characteristics such as relatively low molecular mass, reversibility and generation of stable redox forms due to their nature of excellent electron transfer mediator3. Herein, we report electrochemically simple synthetic approach, capable of yielding random conducting copolymer possessing both polypeptide side chains and ferrocene units. While polypeptide segments created a fascinating biocompatible environment for enzyme immobilization as well as enabling covalent attachment of the enzyme, the ferrocene units improve biosensor performance without any leaching. Alcohol oxidase was used as a model enzyme was immobilized onto the proposed matrix covalently in the presence of glutaraldehyde as the crosslinking agent. The intermediates and final conducting copolymer before and after enzyme deposition were fully characterized. The designed biosensor combining the advantages of each component was tested as the ethanol sensing system and the biosensor design becomes a promising complementary method for ethanol analyisis in alcoholic beverages. The fabricated sensor offers fast response time (9 s), wide linear range (0.17 mM and 4.25 mM) and low detection limit (0.28 mM) with high sensitivity (12.52 µAmM-1cm-2). Kinetic parameters KMapp and Imax were determined as 2.67 mM and 2.98 µA, respectively. The applicability of the biosensor in analyzing ethanol content in real alcoholic samples was also tested and the results approves reliability and accuracy of the biosensor. References 1 A. Ramanaviciene et al. Polym. Chem., 2013, 4, 2206 2 R. Obeid et al. Biomacromolecules, 2011, 12, 3797. 3 J. Qiu et al. Anal. Biochem., 2009, 385, 264.

Synthesis and characterization of conducting polymers containing polypeptide and ferrocene side chains as ethanol biosensors

251st National Meeting (2016)

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