A low cost quantitative analysis methodology was developed through analysis of TLC plate images using Microsoft Paint software. Urinary creatinine was chosen as a model analyte as it is an important marker in a variety of medical tests. Standard and samples containing creatinine were spotted onto a TLC plate and the plate was allowed to develop using the mixture of butanol, ammonium hydroxide, and water. After staining with Iodine vapor, the TLC plate was scanned using an office scanner. The Red-Green-Blue (RGB) values of each spot were recorded using Microsoft Paint. This research found that the Green value gave the most useful working range and sensitivity for detection of creatinine. Analysis of urine samples using a calibration graph obtained from the Green values produced similar results as compared to HPLC.

Thin layer chromatography-digital image analysis (TLC-DIA) for quantitative determination of creatinine

251st National Meeting (2016)

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