Citral is a terpene compound found in the essential oils of lemongrass and other herbs. This study investigates the protective and cytotoxic effects of citral on HaCaT skin cells and M624 melanoma cells, respectively. Cells were cultured and exposed to varying concentrations of citral for three hours. Cell viability following treatment was determined using a clonogenic assay procedure. Bradford analysis was performed following cell lysis. The induction of apoptosis was measured using a caspase-3 colorimetric assay. Results showed that citral has significant toxicity to M624 melanoma cells (IC50- 0.631 mM) and little to no toxicity to normal HaCat cells (IC50- 2.53 mM).

Effects of citral on caspase-3 activation in M624 and HaCaT cells

251st National Meeting (2016)

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