Ever-increasing demand for immunoassays with higher sensitivity is a constant challenge for immunoassay diagnostics. The need for earlier detection and identification of retroviruses like HIV and HCV, the monitoring of short-lived biomarkers like cardiac TnI, and the post-surgery evaluation of TSH levels from a thyroidectomy require immunoassays to detect the desired analyte at very low physiological concentrations. One approach is to develop reagents that emit more relative light units (RLUs) so smaller levels of analyte can be observed. The Abbott ARCHITECT® platform of immunoassay analyzers utilize chemiluminescent acridinium conjugates which emit light when treated with a pretrigger/trigger sequence. Seeking reagents that could generate greater RLUs we prepared and evaluated a new series of pyrroloacridinium reagents to determine their capacity and utility as chemiluminescent reagents.

Pyrroloacridiniums as chemiluminescent reagents for immunoassay diagnostics

251st National Meeting (2016)

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