In the initial stage of manufacturing process, transient transfection in a miniaturized, parallel  bioreactor system can provide the most rapid and cost effective method to generate and screen biopharmaceutical candidates.  In this project, we aim to utilize an AMBR high-throughput microbioreactor system to screen for a fed-batch monoclonal antibody bioprocess that rapidly achieves IgG production using novel DNA-based transfection reagents. With the disposable bioreactors controlled by an automated workstation, design of experiments (DOE) was performed to optimize cell growth, establish reagent cytotoxicity, and explore reagent chemistry. Viable cell density, cell viability, and nutrient concentration were recorded and monitored during the cell culture. Transfection efficiency was quantified by expression level of eGFP through flow cytometry. The harvested IgG concentration was measured by HPLC and compared to the other commercial liposomal and polycationic transfection reagents. Our study demonstrates the utility of the AMBR high-throughput microbioreactor system to facilitate the process development of CHO cell transfection.  <br/>

High-throughput microbioreactors for CHO cell transfection process development

251st National Meeting (2016)

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