There is opportunity for improvement in the analytical techniques used for harvest step development. During the harvest of Chinese Hamster Ovary (CHO) cells, innate run-to-run variability can negatively affect filter capacity and in extreme cases result in filter fouling. During the harvest of E. coli cells, cell lysis via homogenization generates a feed stream that requires a flocculation step to enable centrifugation. This poster presents an evaluation of capturing CHO and E. coli harvest-related particle-size data through the use of Dynamic Light Scattering (DLS) and Focused Beam Reflectance Measurement (FBRM) technologies. Particle-size evaluation of CHO cell culture fluid, CHO harvested cell culture fluid, and E. coli homogenate streams, enabled by these technologies, has lead to potential methods for early filter fouling detection and flocculation characterization for CHO and E. coli harvests, respectively.

Particle size evaluation for improved CHO and E. coli harvest operations

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.