A series of High Performance Liquid Chromatography (HPLC) studies were performed on sweet steviol glycosides isolated from the leaves of Stevia rebaudiana including the Joint Expert Committee on Food Additives (JECFA) compounds namely rebaudioside A, steviolbioside, stevioside, rubusoside, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside F, and dulcoside A. Using Reversed-Phase (RP) HPLC method, individual retention times and area for various ent-kaurane diterpene glycosides of S. rebaudiana have been determined at various temperatures from 20 to 79oC under three different pH 2.4, 2.6, and 2.8 at various UV absorbance. HPLC results suggested that temperatures between 40 and 60oC would be ideal for better separation of steviol glycosides under pH 2.4 at 200 nm.

Reversed-phase high performance liquid chromatography (HPLC) studies of the sweet diterpene glycosides isolated from Stevia rebaudiana bertoni

251st National Meeting (2016)

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