Disulphide bond reduction is a significant issue for manufacturing processes of therapeutic monoclonal antibodies due to its intrinsic property to the functional structure of immunoglobulin.  Several mitigation strategies to prevent or re-oxidize disulphide bond have been reported in the literature.  In this presentation, an innovative methodology utilizing charged depth filtration processes to re-oxidize partially reduced IgG1 is described.  We will summarize our studies comparing different filter types, throughputs and feedstreams with varying partially reduced IgG1 and show that the oxidization kinetics is strongly correlated to the filter throughput and feedstream.  Though the oxidization mechanism is not yet fully understood, our preliminary results show that re-oxidization is related to trace metals from the depth filters.<br/>

Re-oxidization of partially reduced monoclonal antibody by charged depth filters

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.