A matrix material for protein purification based on beads produced from nanocellulose from the green algae Cladophora has been developed. The beads were spontaneously formed during oxidation of the nanocellulose with NaIO4 in a one-pot procedure under aqueous conditions, providing 2,3-dialdehyde cellulose (DAC) beads with an average size of around 15 µm. Degrees of oxidation up to 100% (i.e 12.5 mmol aldehyde per gram of cellulose) was achieved and at high degrees of oxidation (&gt;80%) the morphology of the nanocellulose changed drastically and spherical beads were spontaneously formed. A method to increase the size of the formed beads by allowing them to swell in aqueous solutions of dimethylsulfoxide and to permanently maintain their size by cross-linking the beads with diamines in their swollen state was developed. The method provides beads with an average diameter of 30 µm and a specific surface area of 26 m2/g. The cross-linked beads are stable in sodium hydroxide (1 M) providing a material fulfilling the demands of cleaning-in-place with alkaline solutions. The developed matrix material was evaluated with inverse size exclusion chromatography and had a pore structure suitable for separating small proteins and provide good mechanical stability.

Spontaneous formation of 2,3-dialdehyde cellulose (DAC) beads via periodate oxidation and application of the beads as matrix material in protein purification

251st National Meeting (2016)

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