At Amyris, we have built the Automated Strain Engineering (ASE) and High-Throughput Screening (HTS) pipelines that rapidly accelerate the D-B-T-L cycle at a high-throughput scale using cutting edge synthetic biology tools. With these capabilities, scientists at Amyris can probe multiple metabolic engineering strategies simultaneously, speeding the cycle of strain improvement and parallel investigation of multiple solution sets. Development of these platforms was however a massive undertaking, which required collaborations across multiple disciplines and breaking down the most miniscule strain engineering steps into protocols that could be controlled by software programs and automation scripts. This presentation will cover details of the tool development that enabled Amyris scientists to rapidly iterate through multiple D-B-T-L cycles of the strain engineering process. We will also cover the application of these tools for engineering of metabolic pathways to convert naïve yeast strains into commercial scale production hosts for various products.<br/>

Next generation synthetic biology tools for rapid, high-throughput optimization of metabolic pathways

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.