Recently, a vast amount of work has been carried out in the area of regenerative medicine, focused on generating various types of tissues and using different kinds of organic materials [1]. To create biomimetic tissues a suitable matrix is needed, which provides the structural support. Besides the proper mechanical response, it must be biocompatible and cause minimal inflammation in the human body. In this work, natural sources were used to generate a protein composite biomaterial based on a matrix of Type I collagen extracted from rat tail tendons, with a uniform distribution of nanoparticle extracts from either the plant Tinospora (Tinospora cordifolia) or from Propolis (Própolis), a waxy resin obtained from bee hives. These nanoparticles have different antimicrobial and regenerative properties, which are expect to promote the cell proliferation around the biomimetic tissue [2]. The biomimetic collagen matrix in the form of gel was characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), dynamic mechanical thermal analysis (DMTA), isotermal titration calorimeter (Nano ITC), infrared spectroscopy (FTIR), SDS-Page electrophoresis, scanning electron microscopy (SEM) and atomic force microscopy (AFM) to verify its consistency and properties. The size and morphology of the nanoparticles extracts were analyzed by means of dynamic light scattering (DLS) using Z-average measurements and by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The cytotoxicity of the nanoparticles extracts in fibroblasts was evaluated at different concentrations of the compounds using a cytotoxic essay with Alamar Blue, and although the results were positive for both extracts, cell regeneration was slightly higher for the Propolis nanoparticles. The results of this study show that this composite could become an affordable and effective solution, obtained from natural sources, for the treatment of skin diseases and production of high added value materials from animal and plant wastes. [1] Alvarenga, S., Rojas, M. RepostorioTEC (2011). [2] Abiramasundari, G., Sreepriya, M. Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 5, no. 3 (2014) (pp. 354-363).

Cytocompatible approach toward biomimetic tissues and natural nanoparticles interactions

251st National Meeting (2016)

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