A label-free, sensitive and selective DNAzyme-based quartz crystal microbalance with dissipation (QCM-D) biosensor for rapid detection of toxic metals was developed. This method combines the high selectivity of DNAzymes with the high sensitivity of piezoelectric detection, making it possible for pollution-free detection. Based on this method, detection limit in the nano-molar range could be achieved, which are lower than the EPA regulated levels for toxic metals in environmental samples. This sensor also offers excellent selectivity in the presence of coexisting metal ions. The satisfactory results obtained for demonstrate the feasibility of this approach for online process monitoring. The proposed work extends the application of QCM-D in metal ions sensing by providing high sensitivity and dual-signal measurement. Compared to conventional techniques such as inductively couple plasma (ICP) mass spectrometry, this label-free detection strategy is much more cost effective and smaller in size, providing the possibility for real-time detection of toxic metals. This simple detection strategy could find applications in the on-line industrial process monitoring and quality control that requires the continuous measurements of contaminants.

Highly sensitive and specific detection of toxic metals by biosensors

251st National Meeting (2016)

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