With all apologies to both Johnny Rotten and Steve Cramer, increasing secreted product titers increase the attractiveness of non-chromatographic separations techniques for capture operations.  Here we focus on the use of precipitation as a capture step.  While precipitation doesn’t have the selectivity of chromatography, its selectivity does improve as target species concentrations increase.  Further, precipitation offers advantages in terms of ability to store precipitates indefinitely without significant product loss and to significantly concentrate target/reduce target stream volume on re-dissolution.  Viewed holistically as part of an integrated and optimized process, rather than in isolation, precipitation can offer cost and time savings relative to a chromatography-centric process.  We discuss the application of precipitation-based capture to the purification of monoclonal antibodies and other secreted protein products and illustrate with experimental precipitation data from antibody and other mock target proteins.<br/>

Nevermind chromatography, here’s precipitation

251st National Meeting (2016)

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