Tetrodotoxin (TTX) is a non-protein neurotoxin that is found in several species of animals, such as puffer fish and newts. The focus animal for this project will be the Red-Spotted Newt, <i>Notophtalmus viridescens</i>, which inhabits the northeastern United States. Our laboratory has been making significant progress toward the development and optimization of an enzyme-linked immunosorbent assay (ELISA) to quantify low concentrations of TTX secreted by the newts. Scientists hypothesize that different concentrations of TTX are found during different stages of their life cycle and may also be affected by environmental factors. With an optimized ELISA will be able to quantify the amounts of TTX in different stages of the Red-Spotted Newt’s lifecycle, as well as examine how environmental factors affect the secretion levels.

Optimization of an ELISA for quantifying tetrodotoxin found in red-spotted newt, <i>Notophthalmus viridescens</i>

251st National Meeting (2016)

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Optimization of an ELISA for quantifying tetrodotoxin found in red-spotted newt, Notophthalmus viridescens