Microalgal strains with high oil, or lipid content are of great interest in the search for a sustainable feedstock for the production of biodiesel. Various methods for extraction of lipids have been reported for lipidomic studies. Chloroform/methanol/ water extraction has been reported to extract lipids of all major classes. Matyash et al ( 2008 ) proposed methyl-tert-butyl ether (MTBE) based extraction method for faster and cleaner lipid recovery. For cell disruption, previously it was reported that homogenization of cells is performed better with steel ball than with glass bead milling ( Lee and Fiehn, 2008) . In present work we compared the lipid extraction efficiency of Chloroform/methanol/ water and methyl-tert-butyl ether (MTBE) and also two cell disruption methods steel ball and sonication for microalgae Chlamydomonas reinhardtii . It was observed that number of metabolites extracted with Chloroform/methanol/ water (2:5:2) were higher than methyl-tert-butyl ether (MTBE). Further steel ball and sonication were found be equally effective in disrupting the cell wall for extraction of metabolites.

Metabolomics for understanding the plant chemistry: Comparison of lipid extraction methods for lipid profiling in algae

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.