Farnesyl diphosphate synthase (FPPS) is an important enzymatic catalyst for the formation of farnesyl diphosphate (FPP), a precursor of a family of fifteen-carbon containing natural products, known as the sesquiterpenes. In recent years the structure of many FPPS homologs have been analyzed, but the homolog of FPPS from the archaeon <i>Themoplasma volcanium</i> is not yet known. Our goal is to study the structure of this FPPS homolog to determine any structural adaptations for the thermophilic environment. Current work involves cloning the FPPS gene into a pET28b plasmid with an N-terminal His-tag, followed by expression and purification of the FPPS for protein crystallization trials.

Expression of His-tagged farnesyl diphosphate synthase from <i>Thermoplasma volcanium</i>

251st National Meeting (2016)

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Expression of His-tagged farnesyl diphosphate synthase from Thermoplasma volcanium