Capillary electrophoresis is known for its very high separation efficiencies and peak capacities.  Unfortunately, without an appropriate buffer additive it is impossible to separate chiral compounds for applications from pharmaceuticals to metabolomics.  In this work, a novel set of cationic cyclodextrins with varying functionalities and degrees of derivitization have been used to separate sets of biologically relevant chiral analytes.  The cyclodextrins have been derivitized with trimethylammonium, triethylammonium and tri-n-propyl groups resulting in a significant positive charge.  When cyclodextrins are added into the running buffer, the resulting electroosmotic flow is reversed and chiral separations result from micromolar additive concentrations.  We will present EOF and pH stability studies as well as separation efficiencies for several classes of analytes including amino acids and pharmaceuticals. <br/>

Chiral separations in CE using cationic cyclodextrins as a buffer additive

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.