The detection of cancer protein biomarkers is a quick and effective means of early diagnosis and disease monitoring for improved patient health. Interleukin 6 (IL-6) and Interleukin 8 (IL-8) cancer biomarkers are found in high levels in patients with Cutaneous T cell lymphoma and can be measured as a means to detect cancer. Herein, we report on a rapid microfluidic immunosensor based on nanostructured screen printed carbon electroches coupled to novel multi-labeled magnetic beads, (HRP/MB/Ab2)-PEG, with specially designed polyethylene glycol polymer brushes (PEG). PEG polymer chains prevent non-specific binding and the magnetic bead bioconjugate (MB) aggregation due to repulsive electrostatic forces. PEG was shown to lessen particle aggregation by dynamic light scattering (DLS). The magnetic bead bioconjugate was used for amplified detection in a multi-channeled electrical immunosensor. In this system, the IL-6 and IL-8 antigens were offline captured onto the multi-labeled magnetic beads, (HRP/MB/Ab2)-PEG and allowed to specifically bind to primary antibodies (Ab1) attached to GSH-AuNPs on the electrode array in a microfluidic channel. Catalytic reduction of hydrogen peroxide flowed over the immunoassay complex was used to generate the electrical signal. This immunosensor array gave a detection limit of 10 fg mL-1 and linear range up to 1000fg mL-1 for simultaneous detection of both IL-6 and IL-8 in diluted serum samples. The immunosensor array show great promise for point-of-care cancer diagnosis.

Microfluidic immunosensor arrays based on multi-labeled PEG-coated magnetic beads for detection of 2 cancer biomarker proteins in serum

251st National Meeting (2016)

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