Salmonella is the leading pathogenic bacterium which causes the foodborne infection in human and/or animals. Salmonella typically causes intestinal infection which accompanies the symptoms such as fever, abdominal cramps, and diarrhea. It is occasionally fatal, resulting the morbidity and mortality. For Salmonella infection control, disease assessment, and epidemiological surveillance, it is important to determine the exact serotype among more than 2,500 types. In this study, we developed an oligonucleotide microarray-based analysis system for molecular determination of the commonly isolated Salmonella serotypes in Korea. This microarray system employed a single gene as a novel marker, thiamine synthase H subunit (thiH) gene which was found by comparative genome analysis and have the definite ability of the serotype-level identification. ThiH gene which encodes vital enzyme for survival, has the diverse variant regions for the oligonucleotide capture probes and the common regions for the universal polymerase chain reaction (PCR) primers. Each target serotype of Salmonella can be identified and determined efficiently by the distinctive probe-signal patterns, which can discriminate even the highly closed (related) serotypes. ThiH-based oligonucleotide microarray system can be a useful, robust, and efficient molecular serotype determination tool for Salmonella compared to routine laboratory testing.

Oligonucleotide microarray-based molecular prediction of common Salmonella serotypes

251st National Meeting (2016)

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