251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

American Chemical Society

Downstream Processes

Evaluation of novel adsorptive hybrid filters for impurity removal during biologics purification

Insight into the profile of associated HCP with protein A resin cycle number

Variable surface transport modalities on functionalized chromatographic supports revealed with single molecule spectroscopy

Aggressive development of two recombinant protein production processes to generate a bivalent vaccine

Salt tolerant tangential flow ultrafiltration membranes for protein fractionation

Enabling continuous low pH viral inactivation with integrated in-line conditioning of protein A streams

Evaluating novel harvest methods for high density mammalian cell culture processes

Novel approach for non-chromatographic purification of monoclonal antibodies from cell culture supernatant

Optimized continuous mAb polishing via coupled unit operations

Mechanistic basis for adsorptive selectivity in conventional and dextran-functionalized ion exchangers: Model and experiment

Production of stable high concentration monoclonal antibody solutions with low viscosity by tangential flow ultrafiltration

Residual DNA removal by Benzonase® endonuclease in influenza feedstreams

PD scale realization of an integrated continuous bioprocessing platform

Investigations of alternative clarification methods to obviate protein A on-column precipitation

Leveraging design of experiments to characterize the effects of formaldehyde concentration, incubation temperature and time on influenza A/WS/33 (H1N1) inactivation and process analytics

Selective on-column PEGylation of a therapeutic protein using hydroxyapatite and fluoroapatite adsorbents

Optimization of mAb charge and size variants separation using dual pH/salt gradient elution IEC

Influence of downstream processing on the glycosylation of recombinant anthrax receptor fusion protein transiently expressed in Nicotiana benthamiana

Predicting cation exchange Fab purification using micro-tip chromatography and general rate modelling

Evaluation of preparative ion exchange and multimodal chromatography for separation of charge variants in the mAb purification process

General gradients for preparative chromatography

Technical considerations for an affinity resin discovery and development platform to rapidly address complex separations of diverse therapeutics and vaccines

Development of peptide based affinity media for the purification of monoclonal antibodies: Effect of spacer arms, peptide variants and process conditions

Using microfluidics devices for downstream bioprocess characterisation

Natrix HD-Sb membrane adsorber: A new high-performance purification tool for mAb purification in flowthrough mode

Probing nanoscale effects of ligand density in tentacular ion exchangers using x-ray scattering

Mechanistic modeling for characterization of bioprocess unit operations

Development of efficient cation exchange (CEX) chromatography to remove low and high molecular weight species for commercial manufacturing of a mAb

Mechanistic modeling of antibody transport, adsorption equilibrium, and uptake kinetics in protein A chromatography resins with PEGylated ligand

Application of mechanistic modeling for enabling PAT in process chromatography: Separation of charge variants of mAbs by ion exchange chromatography

Development of robust impurity clearance methods using chromatographic and non-chromatographic techniques

Viral particle purification with osmolyte flocculation

Pre-filtration and process improvements: Enhancing virus filter performance by pre-filtration with surface modified membrane

Mitigating novel ADC impurity challenges through creative approaches: Check the antibody variants

Eco-friendly detergent for viral inactivation

Continuous processing for diafiltration operations in biomanufacturing

Ultrafiltration behavior of highly concentrated monoclonal antibodies

Enabling high-throughput downstream process development: From method screening to process characterization

Evaluation of excipients and cleaning solutions to enhance the performance of Protein A media

Scalability of mechanistic models for ion exchange chromatography

Purification process for heparin using the pH responsive behavior of chitosan

Benzonase® endonuclease for improved primary recovery in an E. coli-based process for Fab production

Investigation of low molecular weight species and implementation of mitigation strategies into large scale biologics manufacturing

Development of a cGMP-compatible purification process for adenovirus purification

Method development for resin selectivity screening in RoboColumn® format

Development of chiral membranes for purifying chiral feed streams

Optimization of bio-molecule separation by combining microscale filtration and design-of-experiment methods

Effects of filtration condition on virus clearance

Maximizing productivity with AEX membrane adsorbers: Impact on robustness and edge of failure

Understanding the impacts of viral clearance artifacts in nanofiltration

Spectral deconvolution of chromatograms with PLS models and calibration-free methods

High capacity polishing chromatography for antibody purification processes

Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Re-oxidization of partially reduced monoclonal antibody by charged depth filters

Development of an affinity precipitation process using protein nanocages as a non-chromatographic alternative to platform therapeutic antibody purification

Enhancing the power of HTPD with empirical interpolation models to predict elution behavior at high protein loads

Assessment of beta glucan leachables originating from cellulose based depth filtration media

Split intein-mediated self-cleaving tag for recombinant protein purification in a mammalian cell expression system

Upstream and downstream solutions for MSCs animal origin-free processing

Progress in development of size exclusion filter paper material for virus removal applications

Thermodynamically based modelling of effects of ethanol and potassium chloride on reversed-phase chromatography

Downstream processes: Past, present and future

Efficient devices for high-resolution membrane chromatography

Evaluation of ligand design for downstream processes:  Application to aggregate removal

Breaking through the productivity barrier: Integration of a novel modular chromatography scaffold and a new resin design to achieve a hyper-productive Protein A capture process

Best of both worlds: High affinity and structural stability in 3D-structured synthetic ligands for protein purification by affinity chromatography

Ultrafiltration for purification of polysaccharide-based vaccines

Knob-hole assembly of bispecific antibodies from half-antibodies

High throughput determination of cleaning solutions to prevent the fouling of an anion exchange resin

Capture resin screening for Bis-scFv purification using high throughput methodologies

Evaluation of cation exchange chromatography resin lot variability and optimization of process conditions for the intermediate purification of a fusion protein

Developing high performance responsive HIC membrane adsorbers

Multiproduct resin reuse for biopharmaceuticals: Application to clinical and commercial manufacturing

Development of challenging aggregate removal step in a bi-specific antibody purification process: A case study

Implementation of a single-use mixer as a point-of-use and disposable heat exchanger in cGMP downstream processing

Investigation of single pass tangential flow filtration as an inline concentration step during cell culture harvest

Leveraging Monte Carlo simulations and mechanistic models to optimize a high concentration UFDF process within facility constraints

Enhancement of viral filtration performance by characterization of the foulant particulates and controlling factors contributing to sub-visible particle formation

Learning from small-scale process characterization studies gone wrong: A case study in chromatography resin lifetime

Downstream Processes

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Evaluation of novel adsorptive hybrid filters for impurity removal during biologics purification