251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

American Chemical Society

Biomolecular & Biophysical Processes

Functional selection of full-length antibodies in the cytoplasm of living bacterial cells

Membrane cholesterol and the adenosine A2a receptor

Influence of the intracellular metabolite profile on outcomes in protein engineering

Bispecific antibody vs. binary mixture: Synergy in the treatment of pertussis

Predicting high-concentration antibody interactions with coarse-grained molecular modeling

Densely modified chymotrypsin-polymer conjugates with increased stability to low pH

Micromachined multielectrode microprobes for choline sensing with an on-probe iridium oxide reference

Extreme thermoacidophiles as biocatalysts for heavy metal recovery: A delicate balance between biooxidation and resistance

SuFEx bioconjugation

Engineered knottin peptide-drug conjugates selectively deliver small molecules to brain tumors in mice

In vivo synthesis of nanoparticles through evolved peptide lipidation

Computationally de novo designed peptide-mediated hetero-oligomeric interactions for modular self-assembly of protein and lipoprotein nanostructures

Creating catenanes with lasso peptides

Modification of bicelles with peptoids to alter edge chemistry

Microrheology and intrinsic viscosities of antibodies

High-throughput conformational epitope mapping by deep sequencing for antibody discovery

Evolving an intrinsically disordered peptide, the β-roll, for biomolecular recognition

Spheroid and tissue assembly via click chemistry in microfluidic flow

N- and C-terminal domains differentially contribute to the structure and function of dystrophin and utrophin tandem calponin-homology domains

Structure-based guidelines for increasing protein conformational stability via PEGylation

Characterization of an engineered, ligand-induced, viral membrane fusion protein

Systemic depletion of serum L-cyst(e)ine with an engineered human enzyme mediates potent induction of ROS and specifically sabotages tumor metabolism

Protein-excipient interaction hotspots: In silico method development and Fab case study

Manipulation and prediction of protein phase behavior: Exploring the influence of PEG-salt mixtures

Adsorption and electron transfer of deca-heme cytochrome (MtrF) studied with atomistic simulations and kinetic Monte Carlo simulation

Platform for constructing, evaluating, and screening bioconjugates on the yeast surface

Directed evolution of yeast peptide receptors: A new platform for low-cost healthcare diagnostic

Understanding short and medium chain volatile ester biosynthesis by AATase activity in yeast

Identification of functional group characteristics and physicochemical properties of Pseudomonas sp. strain ADP biofilm

Measurements of antibody self-association using affinity-capture self-interaction nanoparticle spectroscopy are correlated with complex biophysical properties

Effects of small molecule co-solutes on the viscosity and stability of highly concentrated solutions of monoclonal antibodies

Binary aptamer for instant fluorescent analysis of specific nucleic acids in vitro

Self-assembly of temperature-responsive protein-polymer bioconjugates

Electrospun nanofibers of gelatin/cyclodextrin and their potential application as hydrophobic drug delivery system

Tuning the activity and stability of protein-polymer conjugates by attaching functional polymers

What effect does cleaning and sanitization in place have on protein affinity ligands?

Effect of histatin-5 mutations on proteolytic degradation by Candida albicans secreted aspartic proteases

Whole animal to single cell near infrared fluorescent imaging of antibody drug conjugate metabolism

Peptide ligands bind to electrostatic domains of Abeta

Characterization of protein-functionalized surfaces created by sortase-mediated ligation

Discovery, expression and characterization of novel extremozymes from the deep Red-Sea brine pools

Lessons learned from using phage display to select for fibrin binding peptides

Exploring the space of sense codon reassignment in E. coli

Analyzing the effects of glycosylation on the physicochemical properties and biological activity of antibody Fc regions using a model system

Discovery of highly soluble antibodies prior to purification using affinity-capture self-interaction nanoparticle spectroscopy

Thermodynamics of biomolecule adsorption on calcium oxalate monohydrate crystals

Accelerating pharmaceutical development: Case studies with automated approaches and formulation ‘miniaturization’

Protein conformational array as a process development tool for antibody higher order structure analysis

Improved methods for co-evolving the affinity and stability of antibody fragments specific for amyloid-forming polypeptides

Engineering leave-one-out-GFP based biosensors

Wiring of redox enzymes using a collagen heterotrimer protein

Imaging the oxidation state of peroxiredoxin-2 in living cells

Structural flexibility, hydrophobicity and charge regulation in histidine-rich antimicrobial piscidins underpin membrane disruption examined in real time

Development of transient induced molecular electronic spectroscopy (TIMES) for protein-ligand interactions

Yeast surface display-based method for the directed evolution of optimized self-cleaving intein purification tags

New approaches for high-throughput analysis and engineering of enzymes and protein therapeutics

Dissecting the initial steps of heme uptake between an outer membrane receptor protein of Shigella dysenteriae and hemoglobin by phage display

Effect of culture medium and feeding conditions on antibody fragment secretion system in Escherichia coli

Engineering antibody specificity through multi-dimensional high-throughput screens

Responsive polymer-protein conjugates: From stimulus-induced self-assembly to the treatment of accelerated bone disorders

Linking physical and molecular properties at the single-cell level

Characterization of knob-in-hole assembly variants in support of bispecific antibody development

Inclusion of inter-domain linkers improves the stability and function of fusion proteins in cell-based assays

Novel biocompatible modifications of phage-displayed peptide libraries to generate genetically-encoded libraries of peptide derivatives

Dopa-Fe3+ complexation of mussel adhesive proteins at plaque-substrate interface

Improving the durability of graphene composite sensors for robust biosensors

Biofabrication of protein networks via tobacco mosaic virus-virus like particles (TMV-VLP): 3D scaffold interfaces

Designing custom subcellular organelles in bacteria

Role of redundancy in metabolic networks and its implications in metabolic engineering

Evaluation of pH-responsive hydrogel networks as oral delivery systems for hematologic factor IX

Observing structural changes in biopharmaceutical proteins as a function of concentration

Selection of inhibitory antibodies using next generation high throughput sequencing

Protein nanoparticle conjugates for cancer immunotherapy

Biosynthesis of high-value inositol via in vitro synthetic enzymatic pathways

Point of care device for detection of myeloperoxidase as a wound infection marker

Effect of air-liquid interface in monoclonal antibody aggregate formation

Engineering substrate specificity of chymotrypsin for mass spectrometry based proteomics

Human G protein-coupled receptors expression and signaling in yeast: Design and optimization of the host/protein platform for therapeutic development

Characterization of highly concentrated mAb solution: One step towards the prediction of long-term stability

Cyclic peptide mimics of Aβ binding domain on transthyretin

HaloTag mediated artificial cellulosome assembly on DNA template for efficient cellulose hydrolysis

Dense poly(ethylene glycol) brushes reduce protein adsorption and promote unfolding

Plasma membrane imaging and cancer therapy based on cell surface engineering

Unfolding thermodynamics and β-sheet formation of helical peptides

Solvent-free functional biofluids as a route for retained structure and improved protein stability in nonaqueous environments

Rethinking affibody: Evolving small proteins using data-driven diversification

PET imaging of growth factor receptor expression in cancer using the 45-amino acid Gp2 scaffold

Engineering a novel diagnostic test for tuberculosis using nanoparticle-based detection of a whole blood gene expression signature

Nanobody activation immunotherapeutics that selectively destroy cancer cells

Computational redesign of acyl-ACP thioesterase for medium-chain fatty acid production

How predictive are protein-protein interactions of elevated viscosity for protein solutions

When bad is good: Directed evolution using negative selection can result in alleles with superior properties

Understanding of structural factors for enzyme activity and stability and application to simultaneous enhancement of enzyme activity and organic solvent stability

Biomolecular & Biophysical Processes

No video is available

Functional selection of full-length antibodies in the cytoplasm of living bacterial cells