The use of alkanethiolate-protected gold nanoparticles, specifically C<sub>6</sub> monlayer protected cluster (MPC) network, immobilized in a xerogel matrix was researched. The full developed layer-by-layer scheme incorporates a platinum electrode modified with platinum black (Pt-B), one layer of hydroxymethyl-triethoxy silane (HMTES) incorporating both the MPC network and immobilized uricase enzyme, another subsequent layer of HMTES without either the enzyme or MPC network, an electro-polymerized layer of a luminol/aniline combination, and a layer of HydroThane polyurethane (HPU). Analytical performance of the sensor, both with and without the use of the nanoparticle network, the Pt-Black modification, and the added second layer of HMTES, was determined through amperometric analysis during ongoing uric acid injections into phosphate buffer. The combined effect of the increased surface area with the increased sensitivity, caused by the Pt-B and MPCs, respectively, allowed for reliable uric acid detection across a wide range of concentrations. The demonstrated ability for general and applicable biosensor enhancement through the use of these materials and the overall scheme shall be discussed.

Xerogel layering with monolayer protected cluster networks on platinum black modified electrodes for optimized uric acid biosensing

252nd National Meeting (2016)

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