2D IR spectroscopy has been utilized to directly observe of conformational dynamics of biological systems for both equilibrium and non-equilibrium pathways. Recently, temporal evolution of local structural changes was observed during helix reorganization from a distribution near, but not initially at the equilibrium distribution. Well-chosen probe pairs provide structural information while simultaneously detecting the dynamics in the region where biological function takes place. A systematic study of a variety of 2D IR probes, including cyano- modes, ring modes, and azido- modes will be discussed, focusing on the coupling between different pairings of these vibrational markers as well as their sensitivity to frequency fluctuations resulting from the local environment changes. The associated mechanisms of coupling will be addressed for each coupled pair.

Site-specific vibrational probe pairs for 2D IR studies of biomolecular conformational dynamics

252nd National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.