To identify G protein-coupled receptors involved in juvenile hormone-regulated Vg uptake, we performed RNA interference (RNAi) screen targeting genes coding for GPCRs in the red flour beetle. Out of 112 GPCRs tested, knockdown of 41 GPCRs resulted in a reduction in fecundity. Interestingly, RNAi against only two GPCRs (a Rhodopsin-like receptor and a Dopamine D2-like receptor) led to a significant reduction in Vg accumulation in developing oocytes, without much effect on circulating Vg levels. Functional assays on these two GPCRs showed that JH triggers a dose-dependent inhibition of intracellular cAMP levels in HEK293 cells expressing <i>Tribolium</i> Dopamine D2-like receptor. These data suggest that Dopamine D2-like receptor may be involved in regulating Vg uptake in the red flour beetle.

G protein-coupled receptors involved in vitellogenin uptake into the oocytes

252nd National Meeting (2016)

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