A multitude of important biomolecular processes occur at the cell membrane, including those that involve membrane proteins, lipids, and soluble factors. We have developed a robust array-based approach to simultaneously characterizing multi-factorial biomolecular interactions by integrating Nanodisc technology with multiplexable silicon photonic sensor arrays. Focusing on interactions relevant to blood coagulation, we demonstrate the applicability of this platform to rapidly determine relative binding interactions between a number of coagulation factors with Nanodiscs with varying lipid composition in both the presence and absence of the membrane protein Tissue Factor. By parsing out both lipid and membrane contributions to factor binding and activity we hope to parse out subtle details that provide elegant control over normal blood coagulation as well as dysfunction that leads to both bleeding and clotting disorders.

Multiplexed analysis of biomolecular binding interactions at model cell membrane interfaces using Nanodiscs and silicon photonic sensor arrays

252nd National Meeting (2016)

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