Pierce’s Disease is a devastating disease of grapevines caused by infection with the bacterium Xylella fastidiosa (Xf). In recent years Pierce's Disease has spread rapidly through grapevine populations in California, threatening vineyards in Napa and Sonoma counties. In previous studies, we discovered radicinin, a small organic molecule produced by the endophytic fungus Cochliobolus sp., that inhibits Xf in a dose-dependent fashion. Here, we report the isolation of seven additional fungal strains that inhibit Xf in vitro, including Ulocladium sp. NMR spectroscopy and High-Resolution Mass Spectrometry of the active fractions from Ulocladium sp. indicate a novel, chlorinated secondary metabolite. We report the results of a time-course study to maximize its production, and our efforts at isolating and characterizing this new natural product.

Bioassay-guided isolation and structure elucidation of a natural product inhibitor of Xylella fastidiosa from the endophytic fungus Ulocladium sp

253rd National Meeting (2017)

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